A raiva é uma doença infecto-contagiosa dos mamíferos presente em todos os continentes com exceção da Oceania. É causada por um Lyssavirus e caracterizada como uma encefalite aguda fatal, sendo considerada endêmica no Brasil e a transmissão aos herbívoros ocorre principalmente através de morcegos hematófagos. O objetivo deste experimento foi avaliar a incidência de raiva bovina no Estado de Minas Gerais, assim como métodos de diagnóstico, empregando séries temporais para analisar a tendência e sazonalidade, podendo assim ajustar um modelo da doença em bovinos. Entre 2002 e 2006, amostras de tecido nervoso foram obtidas de bovinos que vieram a óbito com sintomas nervosos e com suspeita clínica de raiva. A imunofluorescência direta (IFD) detectou 40,38% (1.293/3.202) de amostras positivas. Das amostras de tecido consideradas negativas na IFD, 2,67% (51/1.909) foram consideradas positivas utilizando o teste de inoculação em camundongos (IC). Não foi observada sazonalidade e houve uma queda de 0,14 casos positivos ao mês.

Rabies is an infectious contagious disease of mammals present in all continents except Oceania. It is caused by a Lyssavirus and characterized by acute fatal encephalitis. It is considered endemic in Brazil and transmission to herbivores occurs mainly via vampire bats. The purpose of this study was to evaluate the incidence of bovine rabies in the state of Minas Gerais, as well as the diagnostic methods, applying the time series to analyze trend and seasonality in order to adjust a model to disease in cattle. Between 2002 and 2006, nervous tissue samples were obtained from deceased bovines presenting neurological symptoms and clinically suspected of rabies. The direct fluorescent antibody test (FAT) detected 40.38% (1,293/3,202) positive samples. From all FAT negative tissue samples, 2.67% (51/1,909) tested positive in a mouse inoculation test (MIT). No seasonality was observed and positive cases had a decrease of 0.14 per month.

Ventilatory differences between rat strains and genders have been described but the morphology of the phrenic nerve has not been investigated in spontaneously hypertensive (SHR) and normotensive Wistar-Kyoto (WKY) rats. A descriptive and morphometric study of the phrenic nerves of male (N = 8) and female (N = 9) SHR, and male (N = 5) and female (N = 6) WKY is presented. After arterial pressure and heart rate recordings, the phrenic nerves of 20-week-old animals were prepared for epoxy resin embedding and light microscopy. Morphometric analysis performed with the aid of computer software that took into consideration the fascicle area and diameter, as well as myelinated fiber profile and Schwann cell nucleus number per area. Phrenic nerves were generally larger in males than in females on both strains but larger in WKY compared to SHR for both genders. Myelinated fiber numbers (male SHR = 228 ± 13; female SHR = 258 ± 4; male WKY = 382 ± 23; female WKY = 442 ± 11 for proximal right segments) and density (N/mm²; male SHR = 7048 ± 537; female SHR = 10355 ± 359; male WKY = 9457 ± 1437; female WKY = 14351 ± 1448) for proximal right segments) were significantly larger in females of both groups and remarkably larger in WKY than SHR for both genders. Strain and gender differences in phrenic nerve myelinated fiber number are described for the first time in this experimental model of hypertension, indicating the need for thorough functional studies of this nerve in male and female SHR.

Em diversos estudos interdisciplinares em que a Anatomia Vegetal é utilizada, análises quantitativas complementares são necessárias. Geralmente, a avaliação micromorfométrica é feita manualmente e/ou utilizando programas computacionais de análise de imagens não específicos. Este trabalho teve como objetivo desenvolver um programa específico para Anatomia Vegetal quantitativa e testar sua eficiência e aceitação por usuários. A solução foi elaborada na linguagem Java, visando maior mobilidade em relação ao sistema operacional a ser usado. O software desenvolvido foi denominado ANATI QUANTI e testado pelos alunos, pesquisadores e professores do Laboratório de Anatomia Vegetal da Universidade Federal de Viçosa (UFV). Todos os entrevistados receberam fotos para efetuarem medições no ANATI QUANTI e comparar com os resultados obtidos utilizando o software disponível. Os voluntários, através de questionários previamente formulados, destacaram as principais vantagens e desvantagens do programa desenvolvido em relação ao software disponível. Além de ser mais específico, simples e ágil do que o software disponível, o ANATI QUANTI é confiável, atendendo à expectativa dos entrevistados. Entretanto, há necessidade de acrescentar recursos adicionais, como a inserção de novas escalas, o que aumentaria a gama de usuários. O ANATI QUANTI já está em uso nas pesquisas desenvolvidas por usuários na UFV. Por ser um software livre e de código aberto, será disponibilizado na internet gratuitamente.

Complementary quantitative analyses are necessary for several interdisciplinary studies using Plant Anatomy. Generally, micromorphometric evaluation is performed manually and/or using non-specific software for image analyses. This work aimed to develop specific quantitative analysis software for Plant Anatomy and test its efficiency and acceptance by users. The solution was elaborated in the JAVA language, which has a greater mobility in relation to the operating system to be used. The software developed was called ANATI QUANTI and tested by students, researchers and professors of the Plant Anatomy Laboratory of the Universidade Federal de Viçosa (UFV). All the interviewees received photos to measure in ANATI QUANTI and compare with the results obtained using the available software. Through previously formulated questionnaires, the volunteers pointed out the main advantages and disadvantages of the developed software in relation to the currently available software. Besides being more specific, simpler and faster than the currently available software, ANATI QUANTI is reliable, matching the interviewees expectations. However, additional resources need to be added as well as new scales to increase users range. ANATI QUANTI is already being applied in research developed by UFV users. It is a free software and as an open source, it it will be available for free over the internet.